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pbpG [2019-08-12 12:02:14]
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pbpG [2019-08-12 12:02:14]

penicillin-binding protein 2d
Locus
BSU_37510
Isoelectric point
9.39
Molecular weight
71.66 kDa
Protein length
691 aa Sequence Blast
Gene length
2076 bp Sequence Blast
Function
bifunctional glucosyltransferase/ transpeptidase
Product
penicillin-binding protein 2d
Essential
no
Synonyms
ywhE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,849,818 → 3,851,893

    Phenotypes of a mutant

  • a strain lacking all four class A penicillin-binding proteins (ponA pbpD pbpF pbpG) is severely inhibited for L-form switching in the presence of D-cycloserine PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)

    • Protein family

  • N-terminal part: glycosyltransferase 51 family (according to UniProt)
  • C-terminal part: transpeptidase family (according to UniProt)

    • Paralogous protein(s)

  • PbpD, PbpF, PonA

    • Structure

  • 3VMA (PBP1B from E. coli, 29% identity) PubMed

    • Localization

  • cell membrane, the major part of the protein is exposed to the outside (according to UniProt)
  • prespore Link to a summary

    • Expression and Regulation

    Operons

    Genes
    pbpG
    Description
    PubMed

    Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon
  • SigF: sigma factor, in SigF regulon

    • Regulation

  • expressed during sporulation in the forespore (SigF, SigG) PubMed

    • Additional information

  • A ncRNA is predicted between 'pbpG' and 'ywhD' PubMed
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    Biological materials

    Mutant

  • MGNA-A520 (ywhE::erm), available at the NBRP B. subtilis, Japan
  • BKE37510 (ΔpbpG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAACGGGTTCCCCCTTTT, downstream forward: _UP4_TGAAAATAACCCGGCTCCTC
  • BKK37510 (ΔpbpG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAACGGGTTCCCCCTTTT, downstream forward: _UP4_TGAAAATAACCCGGCTCCTC

    • GFP fusion

  • 3510 trpC2 pbpG::pSG5309 (cat Pxyl-gfpa-pbpG1-544) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

    • References

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